Stored at room temperature for three years.
Proteinase K is a non-specific serine protease, and the RNase and DNase activities have been
removed after purification. Proteinase K is active in the presence of SDS or urea as well as in a
wide range of pH, salt concentration and temperature, and can be increased in 1% SDS.
Proteinase K is widely used in the preparation of pulsed electrophoresis chromosomal DNA,
western blot and genomic DNA. The working concentration is usually 50-500g/mL, and the
specific working concentration is determined according to whether the buffer solution
contains SDS, urea, pH, temperature and other factors.
- RNase and DNase are not included;
- Active in denaturant, such as urea and SDS;
- Active in metal chelators, such as EDTA;
- Active in a wide pH range (pH 4-12.5), the best pH range is pH 7.5-8.0;
- Active in a wide range of temperature, generally operating temperature range of 50℃-65℃;
- ddH2O can be dissolved;
- 7.Enzyme activity value: ≥30 U/mg Lyophilized powder.
Method of Inactivation:
After heating at 95℃-98℃ for 10 min, or adding PMSF or DFP inhibitor into the reaction
system, it was deactivated.
- The general storage concentration is 10-20 mg/ml to avoid repeated freeze-thaw;
- Ca2+ can increase the thermal stability of protease K.
The founding team of MegaRobo has over 10 years of industry background for industrial automation. At present, the team has more than 350 people. Driven by independent research and development and technology innovation, MegaRobo has accumulated a number of core technologies with world competitiveness and applied for over 150 invention patents. It co-built the laboratory with Beijing Jiaotong University and Xidian University, and its products have passed ISO9001 Quality Management System Certification. The clients include many well-known enterprises in the industry, such as BGI, Berrygenomics, Wuxi AppTec, RoboCup, Greenland Deepblue, Tesla, Ford, Sinopec, China Aerospace, etc
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